Fig. 3
From: IC261 inhibits the epithelial-mesenchymal transition induced by TGF-β in A549 lung cancer cells
Effects of IC261 on mRNA expression in TGF-β1-induced epithelial mesenchymal transition in A549 cells. A 6-well plate containing serum-free culture medium was seeded with A549 cells and incubated for 6 h. After 4 h of treatment with IC261 (50 and 75 M), the cells were stimulated with TGF-β1 for 24 h. The mRNA expression of N-cad, Vim, and β-cat was analyzed using quantitative reverse transcription polymerase chain reaction (A–D). All data are shown as the mean ± SD (n = 3). #P < 0.0001, compared with the control, and ***P < 0.0001 compared with the TGF-β1 group