Fig. 4
From: IC261 inhibits the epithelial-mesenchymal transition induced by TGF-β in A549 lung cancer cells
Effects of IC261 on TGF-β1-induced phosphorylation of Smad2/3 in A549 cells. A 6-well plate containing serum-free culture medium was seeded with A549 cells and incubated for 6 h. The cells were treated with IC261 (50 and 75 μM) for 4 h, and then stimulated with TGF-β1 for 30 min. The expression levels of phospho-Smad2, phospho-Smad3, Smad2 and Smad3, and GAPDH were analyzed using immunoblotting (A). Protein expression was quantified using ImageJ software and normalized against Smad2/3 expression. The data are presented as the mean ± SD (n = 3). #P < 0.0001 compared with the control, and ***P < 0.0001 and **P < 0.001 compared with the TGF-β1 group