Fig. 5
From: IC261 inhibits the epithelial-mesenchymal transition induced by TGF-β in A549 lung cancer cells
Effects of IC261 on TGF-β1-induced expression of epithelial mesenchymal transition markers. A549 cells were seeded into a coverslip and incubated with serum-free culture medium for 6 h. The cells were treated with IC261 (50 and 75 μM), and then stimulated with TGF-β1 for 42 h. The cells were fixed and stained with antibodies against Vim (upper) and N-cad (lower), followed by incubation with fluorescent dye-tagged secondary antibodies. The nuclei were stained using DAPI. Vim and N-cad were visualized using a confocal microscope