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Fig. 6 | Applied Biological Chemistry

Fig. 6

From: IC261 inhibits the epithelial-mesenchymal transition induced by TGF-β in A549 lung cancer cells

Fig. 6

Effects of IC261 on TGF-β1-induced migration in A549 cells. A549 cells were cultured as a monolayer in a 6-well plate (5.0 × 105 cells/well, plate); the cell cultures were replenished with serum-free culture medium after 24 h. The cells were treated with IC261 (50 and 75 μM) for 4 h, and then stimulated with TGF-β1 for 24 or 48 h. Time-lapse images of the scratch assay for each cell group were recorded at 0, 24, and 48 h using a digital camera (A). The relative wound width was calculated as the ratio of the width of the remaining wound at each time point to the corresponding width at 0 h using ImageJ software (B). Data are presented as the mean ± SD (n = 3). #P < 0.0001 compared with the controls, ***P < 0.0001 compared with the TGF-β1 group

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