Fig. 2From: Piperonal synthase from black pepper (Piper nigrum) synthesizes a phenolic aroma compound, piperonal, as a CoA-independent catalysisGC–MS chromatograms of PnPNS product. A GC–MS analysis of the culture extracts from empty-vector yeast and PnPNS-expressing yeast (YPH499 ΔPAD1 ΔFDC1). Extracted ion chromatograms at m/z 150 are shown. B In vitro recombinant PnPNS assays with 3,4-MDCA. Boiled, Boiled recombinant PnPNS; MDP only, maltose binding protein. C Mass spectra of the synthesized authentic standard and PnPNS productBack to article page