Fig. 5

MiR-516b-5p could directly interact with circ_0001727. A The predicted binding sites for circ_0001727 and miR-516b-5p were presented. B The expression of miR-516b-5p was detected in NSCLC cells after transfection with miRNA NC or miR-516b-5p mimic. C, D The luciferase activity in NSCLC cells co-introduced with miRNA NC or miR-516b-5p mimic and WT-circ_0001727 or MUT-circ_0001727 was detected. E, F Circ_0001727 and miR-516b-5p enrichment were examined in NSCLC cells by RIP assay. G, H The expression of miR-516b-5p was examined in normal tissue samples, NSCLC tissue samples, BEAS-2B cells, and NSCLC cells. I The level of miR-516b-5p was determined in NSCLC cells after transfection with inhibitor NC or miR-516b-5p inhibitor. J MiR-516b-5p expression was measured in NSCLC cells treated with or without propofol. K MiR-516b-5p abundance was tested in NSCLC cells introduced with si-NC, si-circ_0001727, si-circ_0001727 + inhibitor NC, si-circ_0001727 + miR-516b-5p inhibitor, followed by treatment with propofol. *P < 0.05.