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Applied Biological Chemistry
Fig. 7 | Applied Biological Chemistry

Fig. 7

From: Propofol suppresses non-small cell lung cancer progression by modulating circ_0001727/miR-516b-5p/LRRC1 axis

Fig. 7

LRRC1 was directly targeted by miR-516b-5p. A The binding sequence of miR-516b-5p and LRRC1 3′UTR was shown. B–E Dual-luciferase reporter and RIP assays were conducted to confirm the interaction between miR-516b-5p and LRRC1. F, G LRRC1 mRNA and protein abundance in normal and NSCLC tissues were detected. H LRRC1 protein expression was examined by WB in BEAS-2B cells and NSCLC cells. I LRRC1 protein expression was analyzed in NSCLC cells treated with or without propofol. J LRRC1 protein abundance was detected in NSCLC cells transfected with pc-NC or pc-LRRC1. K The expression of LRRC1 protein was determined in NSCLC cells transfected with miRNA NC, miR-516b-5p mimic, miR-516b-5p mimic + pc-NC, or miR-516b-5p mimic + pc-LRRC1, followed by treatment with propofol.

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