Fig. 3
From: Artocarpesin acts on human platelet aggregation through inhibition of cyclic nucleotides and MAPKs
Effects of artocarpesin on fibrinogen binding to αIIb/β3, fibronectin adhesion and VASP and PI3K/Akt phosphorylation. A The flow cytometry histograms on fibrinogen binding. B Effects of artocarpesin on collagen-induced fibrinogen binding (%). C Effects of artocarpesin on collagen-induced fibronectin adhesion. D Effect of artocarpesin on collagen-induced VASP (Ser157) phosphorylation. E Effect of artocarpesin on collagen-induced VASP (Ser239) phosphorylation. F Effect of artocarpesin on collagen-induced PI3K (Tyr458) phosphorylation. G Effect of artocarpesin on collagen-induced Akt (Ser473) phosphorylation. Measurement of fibrinogen binding, fibronectin adhesion, and Western blot was carried out as described in “Materials and Methods” section. The data are expressed as the mean ± standard deviation (n = 4). *p < 0.05, **p < 0.01 versus the collagen-stimulated human platelets