Skip to main content
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: GATA25, a novel regulator, accelerates the flowering time of Arabidopsis thaliana

Fig. 4

Direct activation of flowering response-related genes by GATA25. A Schematic diagram of the GATA25-GR (effector) and reporter constructs used in the transcriptional activation analysis. GATA25 was fused to the glucocorticoid receptor (GR) under the 2ΧCaMV 35S promoter. B GATA25 activates the promoters of GI, FT, CO, and SOC1 in the presence of DEX. Still, adding of the protein synthesis inhibitor cycloheximide at 2 μM completely abolished the DEX-induced GUS activity, indicating that the CHX treatment completely inhibited new protein synthesis. The activity of the GUS in the reporter construct transfected protoplasts with no effector was used as a control. C GATA25 directly induces the expression of flowering response-related genes by DEX activation of GATA25 occurred in the presence of CHX by performing the qRT-PCR analysis. The expression of flowering response-related genes in protoplasts, co-transfected with effector and reporter, treated with CHX was used as a control. Error bars represent the standard deviation (SD) of three biological replicates. Asterisks indicated statistically significant differences compared to Control as determined by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001)

Back to article page