Fig. 3

CF attenuates stem like-cell characteristics in MCF-7/SCs as a BCSC. A Colonies were formed via CF treatment for 10 days (B) CD44⁺/CD24^−/low population measured by FACS after 24 or 48 h of treatment with CF. C ALDH⁺ population after CF treatment for 24 h determined using an Aldefluor assay kit; negative control, diethylaminobenzaldehyde (DEAB). D Migrated cells after 24 or 48 h of treatment with CF by wound healing assay. Phase-contrast micrographs (100 × magnification). E Cell invasion determined using Transwells after 24 h of treatment with CF (0–50 µg/mL). Phase-contrast micrographs (100 × magnification). (F) Stemness markers after 24 h of treatment with CF detected by Western blotting. β-actin was used as the loading control. Data are means ± standard deviation (N = 3). *P < 0.05 for each group compared with the control