Fig. 6.

5-MTP pretreatment reduced LPS-induced inflammation and apoptosis in cultured CFs. A, B and C western blot assay was used for measuring the protein levels of inflammatory cytokine (TNF-α), NLRP3 inflammasome and anti-inflammatory cytokine (IL-10) in cultured CFs (n = 8). D, the change of protein expression level of p-NF-κB in cultured CFs was investigated by western blot assay (n = 8). E and F, the protein levels of pro-apoptotic protein (Bax) and anti-apoptotic protein (Bcl-2) were detected by western blot assay (n = 8). *p < 0.05, **p < 0.01. Data represent the mean ± SD of at least 3 times of separate experiments