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Table 2 HPLC mobile phase gradient conditions

From: Estrogenic activity of freeze-dried silkworm extracts through the activation of estrogen receptors in MCF-7 cells

Time (min)

Mobile phase

A (0.5% acetic acid in water, %)

B (ACN, %)

0

97

3

10

97

3

15

60

40

20

0

100

25

0

100

30

97

3

40

97

3