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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Depressing hsa_circ_0058092 functions an integrated anti-proliferation and anti-motility role in gastric cancer partially through targeting miR-1294/SIX1 axis

Fig. 4

Interactive role between hsa_circ_0058092 depression and miR-1294 inhibition in GC cells. A, B qPCR examined relative expression of miR-1294 in AGS and HGC-27 cells transfected with miR-1294 inhibitor (in-miR-1294) or miR-NC inhibitor (in-miR-NC). C–N AGS and HGC-27 cells transfected with si-hsa_circ_0058092 (#2) or si-NC, si-hsa_circ_0058092 (#2) along with in-miR-1294 or in-miR-NC. C, D Colony formation assay determined the number of colonies formed in 2 weeks. E, F EdU assay measured EdU positive cell rate (%) in Hoechst positive cells. G, H Scratch wound assay displayed relative wound closure (%) after 24 h. I–L Transwell assays tested (I, J) migration rate (%) and (K, L) invasion rate (%) after 24 h. M, N Western blotting detected relative protein expression of E-cadherin and N-cadherin with correction to GAPDH. *P < 0.05, **P < 0.01 and ***P < 0.001

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