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Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Depressing hsa_circ_0058092 functions an integrated anti-proliferation and anti-motility role in gastric cancer partially through targeting miR-1294/SIX1 axis

Fig. 5

Physical interaction between miR-1294 and SIX1. A Schematic diagram showed the wild-type and mutations of the predicted miR-1294-binding sites in SIX1 3’UTR WT and MUT. B, C Dual-luciferase reporter assay determined relative luciferase activity of SIX13’UTR WT and MUT reporter vectors in response to miR-1294 or miR-NC in AGS and HGC-27 cells. D qPCR examined relative mRNA expression of SIX1 in J 41 pairs of Normal and Tumor tissues. E, F Pearson’s correlation coefficient was determined between relative SIX1 mRNA level and miR-1294 and hsa_circ_0058092 levels in 41 Tumor tissues. G Western blotting examined relative protein expression of SIX1 in one pair of randomly selected Normal and Tumor tissues. H, I qPCR and western blotting detected relative mRNA and protein expression of SIX1 in GES-1, AGS and HGC-27 cells. J, K Western blotting measured relative protein expression of SIX1 in AGS and HGC-27 cells transfected with si-hsa_circ_0058092 (#2) or si-NC, si-hsa_circ_0058092 (#2) along with in-miR-1294 or in-miR-NC. **P < 0.01 and ***P < 0.001

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