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Applied Biological Chemistry
Fig. 6 | Applied Biological Chemistry

Fig. 6

From: Depressing hsa_circ_0058092 functions an integrated anti-proliferation and anti-motility role in gastric cancer partially through targeting miR-1294/SIX1 axis

Fig. 6

Reciprocal impact between miR-1294 overexpression and SIX1 hyperexpression in proliferation and motility of GC cells. A, B qPCR and western blotting examined A relative expression of miR-1294 and B relative protein expression of SIX1 in AGS and HGC-27 cells transfected with miR-1294 or miR-NC, pcDNA-SIX1 vector (SIX1) or pcDNA empty vector (pcDNA). C–N AGS and HGC-27 cells transfected with miR-1294 or miR-NC, miR-1294 along with SIX1 vector or pcDNA vector. C, D Colony formation assay determined the number of colonies formed in 2 weeks. E, F EdU assay measured EdU positive cell rate (%) in Hoechst positive cells. G, H Scratch wound assay displayed relative wound closure rate after 24 h. I–L Transwell assays tested I, J migration rate (%) and K, L invasion rate (%) after 24 h. M, N Western blotting detected relative protein expression of E-cadherin and N-cadherin with correction to GAPDH. *P < 0.05, **P < 0.01 and ***P < 0.001

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