Table 2 The antibodies in western blotting and IHC
Antibody | Cat | Dilution ratio | Source |
|---|---|---|---|
SIX1 | 10709-1-AP | 1:1000/1:500 | Proteintech (Wuhan, China) |
E-cadherin | 20874-1-AP | 1:10000 | Proteintech |
N-cadherin | 22018-1-AP | 1:10000 | Proteintech |
GAPDH | 10494-1-AP | 1:50000 | Proteintech |
Ki67 | sc-23900 | 1:100 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) |
HRP-conjugated Goat Anti-Rabbit IgG (H+L) | SA00001-2 | 1:50000 | Proteintech |