Fig. 1

Down-regulation of circGAK in HCC tissues and cells. A Volcano plots indicating dysregulated circRNAs in HCC samples and normal samples from the GSE164803 datasets. B The expression of circGAK in HCC samples and normal samples of GSE164803 datasets. C qRT-PCR detection of circGAK expression in tumor tissues (n = 45) and normal tissues (n = 45) of HCC patients. D qRT-PCR detection of the relative expression of circGAK in advanced stage (III, n = 18) and early-stage (I + II, n = 27) HCC patients. E qRT-PCR analysis of circGAK in the HCC cells (HCCLM6 and MHCC97-H) and normal cells (THLE-2). F Schematic diagram of CircGAK. Identification of the back-splicing junction sites of circGAK by Sanger sequencing. G, H qRT-PCR and I agarose gel electrophoresis assays examining the RNA level of circGAK and β-actin with RNase R treatment in HCCLM6 and MHCC97-H cells. J, K qRT-PCR measuring the level of circGAK, U6, and 18S rRNA in the Nuclear/cytoplasm of HCCLM6 and MHCC97-H cells. Data were expressed as mean ± SD