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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: CircGAK inhibits cell growth, migration, invasion, and angiogenesis of hepatocellular carcinoma via miR-1323/HHIP axis

Fig. 3

The effects of circGAK on miR-1323. A The expression of miR-324-3p and miR-1323 in LIHC (n = 370) and normal (n = 50) by the tool “Pan-Cancer” of the StarBase database. B qRT-PCR detecting the expression of miR-324-3p and miR-1323 in HCC (n = 5) and normal (n = 5) tissues. C Relative expression of miR-1323 in HCC (n = 45) and normal (n = 45) tissues by qRT-PCR. D The level of miR-1323 in advanced stage (III, n = 18) and early-stage (I + II, n = 27) tumor tissues of HCC patients, E as well HCC cells by qRT-PCR. F, G The interaction of circGAK and miR-1323 with Ago2 by RIP assay. H The sequence of wild-type (wt) and mutant (mut) circGAK. I Overexpression efficiency of miR-1323 by qRT-PCR. J, K Verification of miR-1323 as sponged targets of circGAK by dual-luciferase reporter assays. Data were expressed as mean ± SD

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