Skip to main content
Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Isobavachalcone attenuates TNF-α-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells by regulating the NF-κB signaling pathway

Fig. 4

Isobavachalcone decreases phosphorylation of NF-κB p65 in TNF-α-stimulated HUVECs. Immunoblot analysis of phosphorylated NF-κB p65 (A) and degradation of IκB-α (C) in total cell extract were conducted. HUVECs were pretreated with 50 µM isobavachalcone for 30 min and exposed to TNF-α (10 ng/mL) for 4 h (p-p65) and 1 h (IκB-α). Total cell extracts were prepared and analyzed for phosphorylation of p65, total p65, IκB-α and β-Actin. Densitometric analyses results are presented as the relative ratios of phospho-p65 to p65, and IκB-α to β-Actin (B and D). E Expression of phospho-ERK and ERK in TNF-α-stimulated HUVECs co-treated with isobavachalcone for 15 min. (F) Densitometric analyses results are presented as the relative ratios of phospho-ERK to ERK. The bars represent the mean ± SD of three different experiments. ***, p < 0.001 vs. CB; ###, p < 0.001 vs. TNF-α

Back to article page