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Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Isolation, identification, and apoptosis activity of the photosensitizer methyl pheophorbide A from Perilla frutescens leaves

Fig. 2

Morphological changes in U937 and SK-HEP-1 cells by treating with methyl pheophorbide A and light. The cells were seeded with 2 × 105 cells/ml in 48 well plate and incubated for 12 h. After 4 h of treatment with the methyl pheophorbide A, DMSO (the negative control), and 0.1 mM ADCL (the positive control), only the plate of light condition was irradiated at 3250 Lux for 10 min with daylight fluorescent lamps (wavelengths: 440, 550, and 620 nm), PDT-induced cell death was observed. The plate of dark condition was incubated under light blocked condition at all time. The morphology of the cells was observed at 200 × magnification with an IX51 inverted microscope (Olympus, Japan)

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