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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Isolation, identification, and apoptosis activity of the photosensitizer methyl pheophorbide A from Perilla frutescens leaves

Fig. 4

Caspase-3/7 activity by PDT-induced apoptosis following methyl pheophorbide A treatment in U937 (A) and SK-HEP-1 cells (B). Cells were seeded at a density of 2 × 105 cells/ml in 96 well white plate and incubated for 4 h. After 4 h of incubation, the methyl pheophorbide A was treated at a concentration of 0.25, 0.50, 1.00, and 2.00 μg/ml in each well. After inducing photodynamic activity, an equal volume of Caspase-Glo solution reagent was added, plates were shaken for 1 h, and luminescence measured with a GloMax™ multi microplate multimode reader. All experiments were conducted with three replicates, and the results were expressed as mean ± SD (standard deviation). Values were significantly different both by treatment and light with two-way ANOVA (p < 0.05). Different letters represent p-values between light and dark conditions by Post hoc Duncan’s multiple range test

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