Fig. 5

HOXA1 was a miR-515-5p target. A The binding sites of miR-515-5p with HOXA1. B and C Luciferase activity was detected in PCa cells transfected with HOXA1 3ʹUTR-WT or HOXA1 3ʹUTR-MUT and miR-515-5p or miR-NC. D and E HOXA1 mRNA and protein levels were detected in PCa cells transfected with miR-NC or miR-515-5p. F–I The abundances of HOXA1 mRNA and protein were examined in PCa tissues and cells. J Pearson’s correlation analysis assessed the correlation between the levels of miR-515-5p and HOXA1 mRNA in PCa tissues. K and L HOXA1 mRNA and protein levels were estimated in PCa cells transfected with si-NC, si-circTRRAP#1, si-circTRRAP#1 + inhibitor-NC, or si-circTRRAP#1 + miR-515-5p inhibitor. M Pearson’s correlation analysis determined the correlation between the levels of circTRRAP and HOXA1 mRNA in PCa tissues. *P < 0.05