Fig. 1

CircTUBGCP5 was overexpressed in LCa tissues and cells. A Volcano plot of the differentially expressed circRNAs between LCa and normal samples in GSE164803 datasets. B The expression value of circTUBGCP5 in GSE164803 datasets. C The circTUBGCP5 level in LCa (n = 46) and normal (n = 46) tissues by qRT–PCR. D The circTUBGCP5 level in LCa cells (Huh-7 and HCCLM3) and normal cells (THLE-2) using qRT–PCR. E Scheme illustrated the production of circTUBGCP5. PCR primers used to specifically detect circTUBGCP5 by qRT–PCR were indicated by F (Forward primer) and R(Reward primer). The back-splice junction of circTUBGCP5 was identified by Sanger sequencing. F and G CircTUBGCP5 were abundant in the cytoplasm of Huh-7 and HCCLM3 cells, β-actin and U6 were applied as positive controls in the cytoplasm and nucleus, respectively. H and I qRT–PCR examined the RNA level of circTUBGCP5 and β-actin with RNase R treatment in Huh-7 and HCCLM3 cells. Data were expressed as mean ± SD. ***P < 0.001