Fig. 2

circTUBGCP5 enhanced LCa cell proliferation, anti-apoptotic ability, and glycolysis. A The circTUBGCP5 level after transfecting the pLKO.1-puro-sh-NC (sh-NC) vector and pLKO.1-puro-sh-circTUBGCP5 (sh-circTUBGCP5) vector by qRT–PCR. B EdU assay, the scale bar represents 50 µm, C colony formation assay, D Annexin V-FITC/PI assay following knockdown of circTUBGCP5. E Caspase-3 activity, F Caspase-9 activity, G glucose uptake, and H lactate production in transfected with sh-NC or sh-circTUBGCP5 of Huh-7 and HCCLM3 cells. I and J The protein level of c-myc and GLUT1 by western blot assay. Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001