Fig. 5

ACSL4 reversed the function of miR-144-3p in LCa. A Transfection efficacy of ACSL4 overexpression was validated in Huh-7 and HCCLM3 cells via western blot. B and C Cell proliferation, D apoptosis, E Caspase-3 activity, F Caspase-9 activity, G glucose uptake, H lactate production, I and J ACSL4, c-myc, and GLUT1 protein level were detected in Huh-7 and HCCLM3 cells after overexpressing miR-144-3p in the presence or absence of pcDNA-ACSL4. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001