Chemical profile and beneficial effect of standardized extract of Stevia rebaudiana Bertoni leaves on metabolic syndrome in high fat diet streptozotocin-induced diabetic rats

Kamal, Nagham H.; Essmat, Ahmed; El Askary, Hesham I.; El Hefnawy, Hala M.; Wahab, Samia M. Abdel; Meselhy, Meselhy R.

doi:10.1186/s13765-022-00724-8

Applied Biological Chemistry

Table 1 Metabolites tentatively identified in the methanolic extract of S. rebaudiana Bert. leaves using UPLC-Orbitrap HRESI-MS in the negative ion mode

From: Chemical profile and beneficial effect of standardized extract of Stevia rebaudiana Bertoni leaves on metabolic syndrome in high fat diet streptozotocin-induced diabetic rats

Peak no.	R_t (min.)	M⁻	M. Formula	Error (ppm)	MS/MS⁻	UV (nm)	Identification
1	0.66	377.0901	C₁₂H₂₁O ̄	3.5	341.11, 215	–	Sugar alcohol
2	8.71	179.0351	C₉H₇O₄ ̄	0.5	135	333	Caffeic acid
3	8.71	353.0871	C₁₆ H₁₇ O₉⁻	1.87	191, 173, 179, 135	327	3-O-Caffeoylquinic acid (neochlorogenic acid)
4	9.03	707.1814	C₃₂H₃₆O₁₈ ̄	0.127	353	326	Dicaffeoylquinic acid dimer
5	9.23	353.0856	C₁₆H₁₇O₉̄	1.8	173, 179, 191	327	5-O-Caffeoylquinic acid (Chlorogenic acid)
6	9.27	431.1522	C₁₉H₂₇O₁₁ ̄	0.283	299.11, 131.04 149.05	328	Hexosylpentosyl 2-(p-hydroxyphenyl)ethanol
7	9.54	381.0820	C₁₈H₂₁O₉ ̄	1.5	161, 179, 135	328	Ethyl chlorogenate isomer
8	9.63	447.1499	C₁₈H₂₅O₁₀ ̄	2.5	401	328	Benzylhexosylpentoside
9	9.72	431.1913	C₁₉H₂₉O₈ ̄	2.5	385, 223, 205, 153	332	Roseoside
10	9.87	397.1131	C₁₈H₂₁O_{10 ̄}	O.44	191,179, 173	332	p-Sinapoylquinic acid
11	9.93	367.1027	C₁₇H₁₉O₉ ̄	1.14	191, 173, 147	326	5-O-Feruloylquinic acid
12	10.00	537.2020	C₂₆H₃₃O₁₂ ̄	− 0.56	489.12, 375.14, 327.12	325	Hydroxylariciresinol hexoside
13	10.01	611.2481	C₂₆H₄₃O₁₆ ̄	0.22	431, 251	325	Unknown
14	10.15	609.1450	C₂₇H₂₉O₁₆ ̄	0.05	463, 447, 301, 300	335–369	Quercetin hexosyl deoxyhexoside Quercetin -3-O-rutinoside (Rutin)
15	10.31	447.0925	C₂₁H₁₉O₁₁ ̄	1.369	285, 244	344	Luteolin-7-O-glucoside (Cynaroside)
16	10.41	515.1184	C₂₅H₂₃O₁₂̄	0.849	353, 191, 173	329	3,4-di-O-Caffeoylquinic acid
17	10.61	515.1189	C₂₅H₂₃O₁₂̄	3.061	353, 299, 173	328	4,5-di-O-Caffeoylquinic acid
18	10.92	705.3327	C₂₆H₄₁O₉ ̄	2.63	659, 497	308–328	Unknown
19	11.00	677.1502	C₃₄H₂₉O₁₅ ̄	− 0.91	515, 353, 191	329	3,4,5-tri-O-Caffeoylquinic acid
20	11.15	965.4564	C₄₄H₆₉O₂₃ ̄	0.92	803-	nd	Rebaudioside A
21	11.24	849.3761	C₃₉H₆₁O₂₀ ̄	0.42	641, 803	nd	Steviol + 2 Hexose + 4-Methylglucuronide
22	11.41	787.3744	C₃₈H₅₉O₁₇ ̄	1.65	787, 625, 641	nd	Dulcoside A
23	11.54	687.3244	C₃₃H₅₁O₁₅ ̄	0.732	641, 623, 479	nd	Steviol + hexose + 4-methylglucoronid
24	11.55	803.3698	C₃₈H₅₉O₁₈ ̄	0.272	641, 479, 317	nd	Stevioside/Rebaudioside B
25	11.58	641.3172	C₃₂H₄₉O₁₃ ̄	0.767	479, 317	nd	Steviolbioside/rubusoside
26	11.78	359.0766	C₁₈H₁₅O₈ ̄	2.18	344, 329, 286	nd	Centaureidin
27	11.89	335.2229	C₂₀H₃₀O₃ ̄	3.93	317	289	Steviol
28	12.15	479.2653	C₂₆H₃₉O₈ ̄	2.55	317.12	nd	Kaurenoic acid hexoside
29	13.16	295.2273	C₁₈H₃₁O₃ ̄	2.70	277, 195, 171	nd	Unknown

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