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Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Circ_0026359 induces HOXA9 to regulate gastric cancer malignant progression through miR-140-3p

Fig. 2

The effects of circ_0026359 absence on GC cell growth. A The efficiency of circ_0026359 knockdown was detected by qRT-PCR in both AGS and HGC-27 cells. B–I AGS and HGC-27 cells were transfected with sh-circ_0026359 and sh-con, respectively. B CCK-8 assay was carried out to detect cell viability. C Colony formation assay was used to investigate cell colony-forming ability. D and E Cell cycle and apoptosis were analyzed by flow cytometry. F The protein expression of CyclinD1 and Bax was checked by western blot analysis. G and H Cell migration and invasion were evaluated by transwell assays. I Glucose and lactate assay kits were employed to detect glucose uptake and lactate production, respectively. *P < 0.05

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