Fig. 5
From: Circ_0026359 induces HOXA9 to regulate gastric cancer malignant progression through miR-140-3p
The impacts between circ_0026359 knockdown and miR-140-3p inhibitors on GC cell processes. A–H Both AGS and HGC-27 cells were transfected with sh-con, sh-circ_0026359, sh-circ_0026359 + anti-miR-con and sh-circ_0026359 + anti-miR-140-3p, respectively. A MiR-140-3p expression was detected by qRT-PCR. B and C Cell viability and colony-forming ability were detected by CCK-8 and colony formation assays, respectively. D and E Cell cycle and apoptosis were demonstrated by flow cytometry analysis. F The protein levels of CyclinD1 and Bax were determined by western blot analysis. G Cell migration and invasion were evaluated by transwell assays. H Glucose and lactate assay kits were utilized to detect glucose uptake and lactate production, respectively. *P < 0.05