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Fig. 7 | Applied Biological Chemistry

Fig. 7

From: Circ_0026359 induces HOXA9 to regulate gastric cancer malignant progression through miR-140-3p

Fig. 7

HOXA9 overexpression attenuated the effects of miR-140-3p on GC cell processes. A The efficiency of miR-140-3p mimics in increasing miR-140-3p expression was determined by qRT-PCR. B–I AGS and HGC-27 cells were transfected with miR-con, miR-140-3p, miR-140-3p + pcDNA or miR-140-3p + HOXA9. B HOXA9 expression was detected by western blot analysis. C and D Cell viability and colony-forming ability were detected by CCK-8 assay and colony formation assay, respectively. E and F Cell cycle and apoptosis were demonstrated by flow cytometry analysis. G The protein levels of CyclinD1 and Bax were determined by western blot analysis. H Cell migration and invasion were evaluated by transwell assays. I Glucose and lactate assay kits were utilized to detect glucose uptake and lactate production, respectively. *P < 0.05

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