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Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Regulatory effect of peroxiredoxin 1 (PRDX1) on doxorubicin-induced apoptosis in triple negative breast cancer cells

Fig. 3

Deletion of peroxiredoxin 1 (PRDX1) increased the apoptosis of MDA-MB-231 cells. A Green fluorescent protein (GFP) expression was detected by fluorescence microscopy. B The transfection rate of lentivirus was observed by flow cytometry. C The expression of peroxiredoxins (PRDXs) was detected by western blot analysis. D A 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect the cell survival rate after 12 h of DOX treatment. E Mock MDA-MB-231 cells and shPRDX1 MDA-MB-231 cells were treated with DOX at concentrations of 2 μM and 5 μM, and apoptosis was detected by immunofluorescence detection of Annexin V-PE

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