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Fig. 4 | Applied Biological Chemistry

Fig. 4

From: Regulatory effect of peroxiredoxin 1 (PRDX1) on doxorubicin-induced apoptosis in triple negative breast cancer cells

Fig. 4

Peroxiredoxin 1 (PRDX1) knockout promoted doxorubicin (DOX)-induced mitochondrial damage in MDA-MB-231 cells. A Flow cytometry was used to detect intracellular reactive oxygen species (ROS) level after DOX treatment. B The level of ROS in the mitochondria was detected by immunofluorescence microscopy. C Mock MDA-MB-231 cells and shPRDX1 MDA-MB-231 cells were treated with DOX at concentrations of 2 μM and 5 μM, and the mitochondrial membrane potential was detected by immunofluorescence detection of JC-1. D The expression levels of Bad, Bcl-2, C-Cas9, C-Cas7, pro-Cas3, and C-Cas3 proteins were detected by western blot analysis. E The expression levels of Bad, Bcl-2, C-Cas9, C-Cas7, and C-Cas3/pro-Cas3 proteins were statistically analyzed

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