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Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Regulatory effect of peroxiredoxin 1 (PRDX1) on doxorubicin-induced apoptosis in triple negative breast cancer cells

Fig. 5

Inhibition of reactive oxygen species (ROS) reversed peroxiredoxin 1 (PRDX1)-mediated apoptosis in MDA-MB-231 cells. A The level of reactive oxygen species (ROS) species was detected by flow cytometry. B ROS in the mitochondria was detected by fluorescence microscopy. C The mitochondrial membrane potential was observed by fluorescence microscopy. D Apoptosis was observed by fluorescence microscopy. E The expression level of Bad, Bcl-2, C-Cas9, C-Cas7, pro-Cas3, and C-Cas3 proteins were detected by western blot analysis. F The expression levels of Bad, Bcl-2, C-Cas7, pro-Cas3, and C-Cas3 proteins were statistically analyzed

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