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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Production of quinolone derivatives in Escherichia coli

Fig. 2

A HPLC profile of the reaction products from E. coli harboring pA-pqsD-pqsA and pE-pE-pqsE-pqsBC; B HPLC profile from E. coli harboring pC-pqsD-pqsA and pE-pE-pqsE-pqsBC. Anthranilate (200 μM) was supplied to the culture medium. S, anthranilate; P1, P2, and P3 are MHQ, DHQ, and HHQ, respectively. P4 is likely a metabolite derived from acetylation of E. coli metabolites by chloramphenicol acetyltransferase encoded by pACYCDuet-1 vector. When pCDEDuet-1 vector instead of pACYCDuet-1 was used, the P4 was not observed (B)

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