Table 1 Plasmids and strains used in the present study
From: Production of quinolone derivatives in Escherichia coli
Plasmids or E. coli strain | Relevant properties or genetic marker | Source or reference |
|---|---|---|
Plasmids | ||
pACYCDuet-1 | P15A ori, Cmr | Novagen |
pACYCDuet-1-H | Both T7 promoter of pACYCDuet-1 were replaced by promoter H10 | This study |
pCDFDuet-1 | CloDE13 ori, Strr | Novagen |
pETDuet-1 | Both T7 promoter of pETDuet-1 were replaced by promoter C4 | Novagen |
pETDuet-1-C | f1 ori, Ampr | Novagen |
pColaDuet-1 | ColA ori, Kanar | Novagen |
pA-pqsD-pqsA | pACYCDuet + pqsD and pqsA from Pseudomonas aeruginosa | [18] |
pC-pqsD-pqsA | pCDFDuet + pqsD and pqsA from Pseudomonas aeruginosa | [18] |
pA-H-pqsD-pqsA | pACYCDuet-H + pqsD and pqsA from Pseudomonas aeruginosa | This study |
pE-pqsE-pqsBC | pETDuet + pqsE, pqsB and pqsC from Pseudomonas aeruginosa | This study |
pE-C-pqsE-pqsBC | pETDuet-C + pqsE, pqsB and pqsC from Pseudomonas aeruginosa | This study |
pCol-trpE | pColaDuet + trpE from E. coli | This study |
pCol-aroG-trpE | pCDFDuet + aroG from Escherichia coli in the first multiple cloning site (MCS1) + trpE from E. coli in the second MCS (MCS2) | This study |
pCol-aroGf-trpE | pCDFDuet + aroGf from E. coli in MCS1 + trpE in MCS2 | This study |
Strains | ||
DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK +) phoA supE44 λ– thi-1 gyrA96 relA1 | Novagen |
BL21 (DE3) | F− ompT hsdSB(rB− mB−) gal dcm lon (DE3) | Novagen |
B-trpD | E. coli BL21 (DE3) deleted in anthranilate phosphoribosyl transferase domain of trpD | [18] |
B-H1 | E. coli BL21 (DE3) harboring pC-pqsD-pqsA and pE-C-pqsE-pqsBC | This study |
B-H2 | E. coli BL21 (DE3) harboring pA-H-pqsD-pqsA and pE-C-pqsE-pqsBC | This study |
B-H3 | E. coli BL21 (DE3) harboring pA-pqsD-pqsA and pE-pqsE-pqsBC | This study |
B-H4 | E. coli BL21 (DE3) harboring pA-pqsD-pqsA and pE-C-pqsE-pqsBC | This study |
B-H5 | E. coli BL21 (DE3) harboring pC-pqsD-pqsA, pE-C-pqsE-pqsBC, pCol-trpE | This study |
B-H6 | B-trpD harboring pC-pqsD-pqsA, pE-C-pqsE-pqsBC, pCol-trpE | This study |
B-H7 | B-trpD harboring pC-pqsD-pqsA, pE-C-pqsE-pqsBC, pCol-aroG-trpE | This study |
B-H8 | B-trpD harboring pC-pqsD-pqsA, pE-C-pqsE-pqsBC, pCol-aroGf-trpE | This study |