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Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Circ-NT5C2 stimulates FZD4 expression to promote the malignant progression of osteosarcoma by targeting miR-488-3p

Fig. 2

Circ-NT5C2 facilitated cell proliferation and inhibited cell apoptosis of OS cells. AB The overexpression and knockdown transfection efficient of circ-NT5C2 overexpression plasmid and siRNAs was detected by qRT-PCR in SW1353 cells and U2OS cells, respectively. CQ SW1353 cells were transfected with Vector or circ-NT5C2, and U2OS cells were transfected with si-con or si-circ-NT5C2#2. CJ The viability, colony-forming ability and DNA synthesis ability of SW1353 cells and U2OS cells were measured by CCK-8 assay CD, colony formation assay EG and EdU assay HJ. KM The cell apoptosis of SW1353 cells and U2OS cells was detected by flow cytometry. NQ Activity Assay Kits were used to test the Caspase3 and Caspase9 activity. **P < 0.01 and ***P < 0.001

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