Fig. 4

Circ-NT5C2 was physically interacted with miR-488-3p via target binding. A The number of miRNAs that were predicted as targets for circ-NT5C2 in circBank and CircInteractome online software was showed in Venn diagram. B The expression of miR-346, miR-488-3p, miR-593-3p, miR-654-3p, miR-1182 and miR-1231 was measured by qRT-PCR in 6 pairs of OS tissues randomly selected. C–D The expression of miR-346, miR-488-3p, miR-593-3p, miR-654-3p and miR-1182 was determined by qRT-PCR in SW1353 cells transfected with Vector or circ-NT5C2, and U2OS cells transfected with si-con or si-circ-NT5C2#2. E The binding sites of miR-488-3p and circ-NT5C2 WT or MUT Vector were showed. F–G Dual-luciferase reporter assay was performed to determine the luciferase activity in SW1353 cells and U2OS cell transfected with circ-NT5C2 WT/MUT reporter vector and miR-488-3p or miR-NC. H–I RIP assay and qRT-PCR were conducted to detect the enrichment of circ-NT5C2 and miR-488-3p. J–K The expression of circ-NT5C2 was measured by qRT-PCR in 47 pairs of OS tissues as well as hFOB 1.19, SW1353 cells and U2OS cells. **P < 0.01 and ***P < 0.001