Fig. 7

MiR-488-3p regulated OS cell functions via targeting FZD4. A The knockdown and overexpression transfection efficiency of miR-488-3p inhibitor and mimic was separately confirmed by qRT-PCR in SW1353 cells and U2OS cells. B The knockdown and overexpression transfection efficiency of si-FZD4 and FZD4 overexpression plasmid was verified by WB in SW1353 cells and U2OS cells. C–R in-miR-NC, in-miR-488-3p, in-miR-488-3p + si-NC or in-miR-488-3p + si-FZD4 was transfected into SW1353 cells, while miR-NC, miR-488-3p, miR-488-3p + pcDNA or miR-488-3p + FZD4 was transfected into U2OS cells. C–H CCK-8 assay C, D, colony formation assay E–F and EdU assay G–H were performed to detect the cell proliferation. I–J The cell apoptosis was detected by flow cytometry. K–L Activity Assay Kits were used to test the Caspase3 and Caspase9 activity. M–P The migrated and invaded cells was measured by transwell assay. Q–R The protein expression of E-cadherin and N-cadherin was detected by WB. **P < 0.01 and ***P < 0.001