Applied Biological Chemistry

Table 1 Primers sequences used for PCR

From: Circ-NT5C2 stimulates FZD4 expression to promote the malignant progression of osteosarcoma by targeting miR-488-3p

Name		Primers for PCR (5’–3’)
circ-NT5C2	Forward	TGCCAGTCAAGTGATGCGTT
circ-NT5C2	Reverse	TGGAACTGATGTCTGGACGC
NT5C2	Forward	TACCTACACAGGGCCCCTAC
NT5C2	Reverse	CCACCCTTGCCGTTTCTTTG
miR-346	Forward	GCCGAGTGTCTGCCCGCAT
miR-346	Reverse	CTCAACTGGTGTCGTGGAG
miR-488-3p	Forward	GCCGAGTTGAAAGGCTATT
miR-488-3p	Reverse	CTCAACTGGTGTCGTGGAG
miR-593-3p	Forward	GCCGAGTGTCTCTGCTGGG
miR-593-3p	Reverse	CTCAACTGGTGTCGTGGAG
miR-654-3p	Forward	GCCGAGTATGTCTGCTGACC
miR-654-3p	Reverse	CTCAACTGGTGTCGTGGAG
miR-1182	Forward	GCCGAGGAGGGTCTTGGGAT
miR-1182	Reverse	CTCAACTGGTGTCGTGGAG
miR-1231	Forward	GCCGAGGTGTCTGGGCGGA
miR-1231	Reverse	CTCAACTGGTGTCGTGGAG
FZD4	Forward	CAGCAGGACTCAAATGGGGT
FZD4	Reverse	CAATGGTTTTCACTGCGGGG
GAPDH	Forward	GACAGTCAGCCGCATCTTCT
GAPDH	Reverse	GCGCCCAATACGACCAAATC
U6	Forward	CTCGCTTCGGCAGCACA
U6	Reverse	AACGCTTCACGAATTTGCGT

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