Biochemical and histopathological studies of sulfonylurea derivative as a new chemotherapeutic agent against liver cancer in free- and nano-coated forms

Applied Biological Chemistry

Table 6 Rate of DNA damage in liver tissues of male rats exposed to different treatment using comet assay

Treatment	No. of cells		Class^¥ of comet				DNA damaged cells (mean ± SEM)
Treatment	Analyzed*	Total comets	0	1	2	3	DNA damaged cells (mean ± SEM)
HePG2 (-ve)	300	28	273	19	7	2	9.30 ± 0.89^c
HePG2 (+ ve)	300	75	226	34	23	18	24.93 ± 1.15^a
5-Fluorouracil	300	68	233	31	17	20	22.59 ± 1.20^ab
HePG2 (coated 3)	300	51	250	21	19	11	16.94 ± 0.58^b
HePG2 (non-cated 3)	300	59	242	19	26	14	19.61 ± 0.67^b

(^¥): Class 0 = no tail; 1 = tail length < diameter of nucleus; 2 = tail length between 1 and 2X the diameter of nucleus; and 3 = tail length > 2X the diameter of nucleus
(*): No of cells analyzed were 100 per an animal