Table 3 The recovery results of seven metabolites from C18 cartridge according to the ratio of methanol (MeOH) in water
Fraction | Recovery (%) | ||||||
|---|---|---|---|---|---|---|---|
IN-A5760 | IN-F4106 | IN-QEK31 | IN-QZY47 | IN-TMQ01 | IN-UNS90 | IN-UJV12 | |
Loading | –a | – | – | – | – | – | – |
1 (MeOH 10%) | – | – | – | – | – | – | 13.2 |
2 (MeOH 10%) | 97.8 | – | – | – | – | – | 67.5 |
3 (MeOH 20%) | 18.7 | – | – | 19.9 | – | 88.1 | – |
4 (MeOH 20%) | – | 50.4 | – | 73.9 | – | 9.6 | – |
5 (MeOH 30%) | – | 38.7 | – | – | 40.6 | – | – |
6 (MeOH 30%) | – | – | – | – | 55.4 | – | – |
7 (MeOH 40%) | – | – | 42.0 | – | – | – | – |
8 (MeOH 40%) | – | – | 58.5 | – | – | – | – |
9 (MeOH 50%) | – | – | – | – | – | – | – |
10 (MeOH 50%) | – | – | – | – | – | – | – |
Total | 116.5 | 89.1 | 100.5 | 93.8 | 96.0 | 97.7 | 80.7 |