Diatom frustule silica extracted from Melosira nummuloides ameliorates acetaminophen-induced acute liver injury in mice

Applied Biological Chemistry

Table 1 The primer sequences and annealing temperature for qPCR

Gene	Direction	Sequences	NCBI Number	Annealing temperature
TNF-α	Forward	TTGTCTACTCCCAGGTTCTCTT	NM_001278601.1	58
TNF-α	Reverse	ACTTTCTCCTGGTATGAGATAGC	NM_001278601.1	58
IL-1β	Forward	TGACTCATGGGATGATGATGATAAC	NM_008361.4	55
IL-1β	Reverse	TGAGGTGGAGAGCTTTCAG	NM_008361.4	55
IL-6	Forward	CTCTCTGCAAGAGACTTCCAT	NM_001314054.1	58
IL-6	Reverse	CCGACTTGTGAAGTGGTATAG	NM_001314054.1	58
β-actin	Forward	CCACCAGTTCGCCATGGAT	NM_007393.5	56
β-actin	Reverse	CCACGATGGAGGGGAATACA	NM_007393.5	56