Fig. 4
CerS4 and CerS6 play an important role in PAL-induced β-cell death. A Representative western blots of indicated proteins after 0.5 mM PAL treatment in CerS4- or CerS6-overexpressed MIN6 cells. B Nitrite and C TNF-α levels were measured after 0.5 mM PAL treatment in CerS4- or CerS6-overexpressed MIN6 cells (n = 3). Values are expressed as mean ± SEM compared to control group; **P < 0.01, ***P < 0.001 and compared to PAL-treated group; #P < 0.05, ##P < 0.01. All experiments were performed in triplicate. CerS ceramide synthase, PAL palmitate, TNF-α tumor necrosis factor-alpha, Bcl2 B-cell lymphoma 2, eIF2α eukaryotic translation initiation factor 2A, iNOS inducible nitric oxygen synthase; PERK protein kinase R-like endoplasmic reticulum kinase, Con control (untreated)