Table 1 Primers used for real-time PCR
Gene | Primer sequences |
|---|---|
CerS1 (for mouse) | F: 5ʹ- TCCATCTATGCCACCGTGTA -3ʹ R: 5ʹ- GCGTAGGAAGAGGCAATGAG -3ʹ |
CerS2 (for mouse) | F: 5ʹ- TCTGCATGACGCTTCTGACT -3ʹ R: 5ʹ- GATGGCGAACACAATGAAGA -3ʹ |
CerS3 (for mouse) | F: 5ʹ- AAGCATTCCACAAGCAAACC -3ʹ R: 5ʹ- GCCGAATCCTAAGCCATCTT -3ʹ |
CerS4 (for mouse) | F: 5ʹ- TGCGCATGCTCTACAGTTTC -3ʹ R: 5ʹ- CAGAAACTGGCTCGTCATCA -3ʹ |
CerS5 (for mouse) | F: 5ʹ- GCAATCTTCCCATTGTGGAT -3ʹ R: 5ʹ- TCAGGAGAAGGGCATTGAAG -3ʹ |
CerS6 (for mouse) | F: 5ʹ- TTAGCTACGGAGTCCGGTTC -3ʹ R: 5ʹ- TGAAGGTCAGCTGTGAGTGG -3ʹ |
GAPDH (for mouse) | F: 5ʹ- ACTCACGGCAAATTCAACGG -3ʹ R: 5ʹ- ATGTTAGTGGGGTCTCGCTC -3ʹ |