Fig. 2

The biological role of circPDXK dysregulation in OVCA cells. A QPCR estimated relative circPDXK expression in SKOV3 and A2780 cells transfected with si-NC, si-circPDXK#1 or si-circPDXK#2. (B-M) SKOV3 and A2780 cells were harvested after transfections of si-NC and si-circPDXK#2. B Cell viability was detected using CCK8 assay by measuring OD values (λ = 450 nm) at different culture durations. C Colony formation was determined using colony formation assay by counting the number of colonies. D DNA synthesis was examined using EdU assay by calculating EdU positive cell rate (%). Hoechst was total DNA dye, EdU was new DNA stain. E, F Transwell assays evaluated migration and invasion abilities by microscopically counting numbers of migrated cells and invaded cells in per field (100 ×). G FCM method and Annexin V-FITC/PI staining showed apoptosis rate (%). H–J Western blotting detected relative protein expression of ki67, Bax and E-cadherin with normalization to β-actin. K–M Glucose uptake, lactate and ATP assay kits separately examined relative glucose uptake, lactate production and intracellular ATP level. **P < 0.01; ***P < 0.001