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Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Circular RNA pyridoxal kinase (circPDXK) involves in the progression of ovarian cancer and glycolysis via regulating miR-654-3p and hexokinase II

Fig. 3

The functional mechanism of circPDXK via directly interacting with miRNA. A Venn diagram showed the potential target miRNAs according to CircInteractome, starBase, and circBank prediction tools. B, C QPCR estimated the relative expression of miR-582-3p and miR-654-3p in SKOV3 and A2780 cells after transfections of si-NC and si-circPDXK#2. D Schematic plot showed the predicted miR-654-3p target sites in circPDXK WT and their mutations in circPDXK MUT. E QPCR estimated relative miR-654-3p expression in SKOV3 and A2780 cells after transfections of miR-654-3p mimic and miR-NC mimic. F, G Dual-luciferase reporter assay determined relative luciferase activity of circPDXK WT and circPDXK MUT report vectors in condition of miR-654-3p or miR-NC overexpression. H, I RIP and qPCR assays confirmed relative RNA enrichment of circPDXK and miR-654-3p in the input and precipitations mediated by anti-Ago2 and anti-IgG. J, K QPCR estimated the relative miR-654-3p expression in (J) tumor tissues and adjacent tissues from OVCA cancer patients (n = 52), and (K) HOSEpiC, SKOV3 and A2780 cells. L Linear correlation coefficient (r) between circPDXK and miR-654-3p levels in 52 OVCA tumors was determined by Pearson correlation analysis. *P < 0.05; **P < 0.01; ***P < 0.001

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