Fig. 4

The weakening effect of miR-654-3p on circPDXK role. A–O SKOV3 and A2780 cells were harvested after single transfections of si-NC, si-circPDXK#2, in-miR-NC, and in-miR-654-3p, and co-transfections of si-circPDXK#2 along with in-miR-NC or in-miR-654-3p. A, B QPCR estimated the relative expression of miR-654-3p. C, D CCK8 assay measured OD values (λ = 450 nm) at different culture durations. E Colony formation assay determined the number of colonies. F EdU assay examined EdU-positive cell rate (%). G, H Transwell assays evaluated numbers of migrated cells and invaded cells per field (100 ×) under a microscope. I FCM method and Annexin V-FITC/PI staining showed apoptosis rate (%). J–L Western blotting detected relative protein expression of ki67, Bax, and E-cadherin with normalization to β-actin. M–O Glucose uptake, lactate, and ATP assay kits separately examined relative glucose uptake, lactate production, and intracellular ATP level. *P < 0.05; **P < 0.01; ***P < 0.001