Fig. 6

Effects of miR-654-3p and HK2 on progression and glycolysis of OVCA cells. A–O SKOV3 and A2780 cells were harvested after transfections of the control pcDNA vector, HK2 overexpression vector, miR-NC, miR-654-3p, and miR-654-3p combined with the control pcDNA vector or HK2 overexpression vector. A, B Western blotting examined the relative protein expression of HK2. C, D CCK8 assay measured OD values (λ = 450 nm) at different culture durations. E Colony formation assay determined the number of colonies. F EdU assay examined EdU-positive cell rate (%). G, H Transwell assays evaluated numbers of migrated cells and invaded cells per field (100 ×) under a microscope. I FCM method and Annexin V-FITC/PI staining showed apoptosis rate (%). J–L Western blotting detected relative protein expression of ki67, Bax and E-cadherin with normalization to β-actin. M–O Glucose uptake, lactate and ATP assay kits separately examined relative glucose uptake, lactate production and intracellular ATP level. *P < 0.05; **P < 0.01; ***P < 0.001