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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Escherichia coli methionine-tRNAi/methionyl tRNA synthetase pairs induced protein initiation of interest (PII) expression

Fig. 1

Schematic depiction of the maintenance of the reading frame for the translational initiation of non-formylated methionine Escherichia coli initiator tRNA (E. coli Met-tRNAi). Structure of single fluorescent methionine E. coli initiator tRNA (fluorescent Met-tRNAi), which initiates the expression of proteins of interest (enhanced green fluorescent protein or Tat). First, a non-formylated group was attached to the free amino group of Met-tRNAi. Met-tRNAi was fluorescent-labeled via a reaction with its amine reactive group. Methionylation via E. coli methionyl tRNA synthetase (EcMRS) charged E. coli Met-tRNAi for incorporation into the proteins of interest in HeLa cells

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