Fig. 4
From: Identification and characterization of drought-induced long noncoding RNAs (DRILs) in rice
Overexpression of the DRILs in rice protoplasts. A Schematic diagram of the transient overexpression constructs of the DRILs. In the constructs, the DRILs are under the GOS2 promoter and PinII terminator. The binary vector was generated through the gateway system. B DRIL1 overexpression and the expression levels of stress marker genes. OsUbi1 was used as an internal control for normalization. C The expression of the putative cis-target genes of DRIL1. Numeric values in Tables indicate fold changes derived from RNA-sequencing with long-term drought stress-treated samples. d, drought; C, control. D DRIL4 overexpression and the expression levels of stress marker genes. E The expression of the putative cis-target genes of DRIL4. Numeric values in Tables indicate fold changes derived from RNA-sequencing with long-term drought stress-treated samples. d, drought; C, control. F Overexpression of DRIL6 and OsDof2. The green bars represent OsDof2 overexpression, and the yellow bars represent DRIL6 overexpression (unpaired Student’s t-test, *P ≤ 0.05, **P ≤ 0.01)