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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: Improved quantification of catechin and epicatechin in red rice (Oryza sativa L.) using stable isotope dilution liquid chromatography-mass spectrometry

Fig. 2

Effect of different extraction temperatures and times on the extraction efficiency of catechin and epicatechin. Area of catechin, epicatechin, catechin-2,3,4-13C3, and epicatechin-2,3,4-13C3 at 30 ℃ A, 50 ℃ B, 70 ℃ C, and 100 ℃ D as extraction time progressed. Catechin (E) and epicatechin (F) stability using L-2-chlorophenylalanine and SIL-IS (catechin-2,3,4-13C3 and epicatechin-2,3,4-13C3) as an internal standard. 100% represents the concentration at 0 h. An increase above 100 means that the catechin and epicatechin content has increased. Percentages under 100 indicate that the catechin and epicatechin content has decreased. Statistical significance was determined by two-way ANOVA followed by Bonferroni post-tests to compare each time point to 0 h (*p < 0.05, ***p < 0.001). All data are expressed as mean value of three experiments ± SD. C catechin, E epicatechin, SIL-IS stable-isotope-labeled internal standard

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